UV/NTG Mutagenesis for Breeding of A High Trehalose-producing Strain of Saccharomyces cerevisiae

doi:10.7506/spkx1002-6300-200921044

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (21 ): 188-191.doi: 10.7506/spkx1002-6300-200921044

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UV/NTG Mutagenesis for Breeding of A High Trehalose-producing Strain of Saccharomyces cerevisiae

ZHANG Chun-ling

College of Power Transmission and Transformation on Technology, Northeast Dianli University, Jilin 132012, China

Received:2009-06-13 Online:2009-11-01 Published:2010-12-29
Contact: ZHANG Chun-ling E-mail:jlzcl@126.com

Abstract

Abstract:

A high trehalose-producing strain of Saccharomyces cerevisiae was bred via combined nitrosoguanidine (NTG)/UV mutagenesis. This strain had good genetic stability and a trehalose content of 19.8% (m/m dry yeast), which revealed the enhancement of 39.43%, 10.0% and 6.45% compared with the original strain, the strain screened by alone UV mutagenesis, and the strain screened by alone NTG mutagenesis, respectively.

Key words: UV, NTG, trehalose, combined mutagenesis, Saccharomyces cerevisiae

CLC Number:

TS201.3

ZHANG Chun-ling. UV/NTG Mutagenesis for Breeding of A High Trehalose-producing Strain of Saccharomyces cerevisiae[J]. FOOD SCIENCE, 2009, 30(21 ): 188-191 https://doi.org/10.7506/spkx1002-6300-200921044

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UV/NTG Mutagenesis for Breeding of A High Trehalose-producing Strain of Saccharomyces cerevisiae

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